GHK is a research compound. It is not approved by the FDA or any regulatory body for human use. This article is for educational and informational purposes only. Nothing here constitutes medical advice. Consult a qualified physician before considering any peptide use.
GHK is typically reconstituted with bacteriostatic water (BAC water). The standard dosage of 1-3 mg (similar to GHK-Cu protocols) is administered once daily via topical or subcutaneous injection. Use our peptide calculator for exact mixing ratios.
How Do You Reconstitute GHK?
GHK is supplied as a lyophilized (freeze-dried) powder that must be reconstituted before use. Proper reconstitution is critical for accurate dosing and maintaining stability.
This guide covers the step-by-step process for mixing GHK with bacteriostatic water to achieve your target concentration.
What Supplies Do You Need?
To reconstitute GHK, you need: the GHK vial (lyophilized powder), bacteriostatic water (BAC water with 0.9% benzyl alcohol), insulin syringes (typically 1mL/100-unit), alcohol swabs, and a clean workspace.
Important: Always use bacteriostatic water — not sterile water or saline. BAC water's benzyl alcohol prevents bacterial contamination, extending the usable life of reconstituted GHK to 4-6 weeks.
Step-by-Step GHK Reconstitution
Step 1: Clean the vial tops of both the GHK and BAC water with alcohol swabs.
Step 2: Draw the calculated amount of BAC water into an insulin syringe. Use our peptide calculator to determine the exact amount.
Step 3: Insert the needle into the GHK vial at an angle, and let the water run down the side of the glass — never spray directly onto the powder as this can damage the peptide bonds.
Step 4: Gently swirl (do not shake) the vial until the powder is fully dissolved. The solution should be clear.
Step 5: Label the vial with the date and concentration. Store at 2-8°C.
Calculate Your GHK Dose
Use our free peptide dosing calculator to get exact reconstitution math and syringe units for GHK.
Open Calculator →How Do You Dose Reconstituted GHK?
After reconstitution, the standard GHK dose is 1-3 mg (similar to GHK-Cu protocols) administered once daily via topical or subcutaneous injection. The number of units on your insulin syringe depends on how much BAC water you added.
Our calculator will tell you exactly how many units to draw for your dose based on your specific reconstitution ratio.
How Do You Store Reconstituted GHK?
Store reconstituted GHK at 2-8°C (standard refrigerator temperature). Use within 4-6 weeks. Keep away from light and temperature fluctuations.
Unreconstituted GHK powder can be stored at -20°C for 12+ months. Once you reconstitute it, the clock starts.
Bottom Line
Reconstituting GHK is straightforward — add BAC water, swirl gently, refrigerate. The key is using the right amount of water for accurate dosing. Use our peptide calculator every time.
Complete Guide
GHK : Benefits, Dosage, Side Effects & Research
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If you're going to research GHK, source matters. These are the suppliers WolveStack has vetted for purity and third-party testing.
Frequently Asked Questions
What is GHK?
GHK (Glycyl-L-Histidyl-L-Lysine) is a Collagen-modulating tripeptide. Endogenous human tripeptide found circulating in plasma; this is the copper-free form of the GHK-Cu complex. It is researched for skin regeneration support, collagen precursor activity, wound healing support.
What is the recommended GHK dosage?
Common dosages: 1-3 mg (similar to GHK-Cu protocols) administered once daily via topical or subcutaneous injection. Cycle length: 4-12 weeks. Half-life: unknown for copper-free form. Use our peptide calculator for exact reconstitution math.
What are the side effects of GHK?
Zero human safety data for copper-free GHK specifically. GHK-Cu shows minimal side effects. Topical irritation possible.
Is GHK safe?
GHK has shown a preliminary safety profile in research. Available as research chemical and cosmetic ingredient. All research should follow appropriate safety protocols.