Research Reference Only — This calculator is a mathematical conversion tool for educational purposes only. It does not constitute medical advice, diagnosis, or treatment. The compounds referenced are research chemicals not approved by the FDA for human use. Never self-administer any substance without supervision from a licensed healthcare professional. See our full disclaimer.
Reconstitution Calculator
Works for BPC-157, TB-500, CJC-1295, Ipamorelin, and any lyophilized peptide
Step-by-Step Instructions
Research use only. These calculations are for educational reference. Peptides are unscheduled research chemicals, not approved medications. Consult a qualified medical professional before use. WolveStack does not provide medical advice.
Common Peptide Reference Doses
Based on community protocols discussed in research forums. Not medical advice.
| Peptide | Common Range | Typical Frequency | Route | Complexity |
|---|---|---|---|---|
| BPC-157 | 200–500 mcg | Once or twice daily | SubQ or IM | Low |
| TB-500 | 2–5 mg | 2× weekly (loading), weekly (maintenance) | SubQ or IM | Low |
| Wolverine Stack | 250 mcg BPC + 2.5 mg TB | BPC daily, TB 2×/week | SubQ | Medium |
| CJC-1295 (no DAC) | 100–200 mcg | 3× daily (pulsed) | SubQ | Medium |
| Ipamorelin | 200–300 mcg | 3× daily (pulsed) | SubQ | Medium |
| Selank | 250–500 mcg | Once or twice daily | Intranasal or SubQ | Low |
| PT-141 | 0.5–2 mg | As needed (1–2 hrs pre) | SubQ | Low |
| AOD-9604 | 300–500 mcg | Once daily (fasted) | SubQ | Low |
| GHK-Cu | 1–3 mg | Once daily | SubQ or topical | Low |
| Epithalon | 5–10 mg | Once daily (cycles) | SubQ or IM | Medium |
Quick Unit Conversion Reference
The mcg → mL math that trips up first-timers.
| If your vial is… | You add 1 mL BAC water | You add 2 mL BAC water | You add 3 mL BAC water |
|---|---|---|---|
| 5 mg vial | 5,000 mcg/mL → 250 mcg = 5 units | 2,500 mcg/mL → 250 mcg = 10 units | 1,667 mcg/mL → 250 mcg = 15 units |
| 10 mg vial | 10,000 mcg/mL → 250 mcg = 2.5 units | 5,000 mcg/mL → 250 mcg = 5 units | 3,333 mcg/mL → 250 mcg = 7.5 units |
| 2 mg vial | 2,000 mcg/mL → 200 mcg = 10 units | 1,000 mcg/mL → 200 mcg = 20 units | 667 mcg/mL → 200 mcg = 30 units |
Calculator FAQ
Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial growth in the vial after you've punctured the stopper. A reconstituted peptide vial can be punctured multiple times safely over 4–6 weeks when stored at 2–8°C in BAC water. Sterile water has no preservative, so a reconstituted vial should ideally be used within 24–48 hours to minimize contamination risk. For multi-use vials, BAC water is the standard choice.
Only in terms of the resulting concentration, which affects how many units you draw per dose. More water = lower concentration = more units per dose. Neither is intrinsically better — the math is what matters. Many people prefer 2 mL per 5 mg vial because it gives round numbers on a U-100 syringe. Use this calculator to find what works for your syringe and dose target.
Both hold 1 mL of liquid, but the graduations differ. A U-100 syringe has 100 units marked on it (each unit = 0.01 mL). A U-50 syringe has 50 units (each unit = 0.02 mL). U-100 syringes are the standard for most peptide protocols because they allow more precise measurement for small doses. The calculator accounts for both — just select yours in the dropdown.
1 mg = 1,000 mcg. So a 5 mg vial contains 5,000 mcg total. When you see peptide doses listed as "250 mcg," that's 0.25 mg — a fraction of the total vial. This matters because vial sizes are typically labeled in mg (e.g., 5 mg, 10 mg) while individual doses are described in mcg (e.g., 250 mcg, 500 mcg). The calculator handles this conversion automatically.
Small air bubbles in subcutaneous (SubQ) injections are generally considered benign — SubQ injections go into fat tissue, not directly into a vein. That said, best practice is to tap the syringe gently and push the plunger slightly to expel visible bubbles before injecting. Air bubbles in intravenous injections are a different matter and pose real risk, but most peptide protocols use SubQ injection into the abdomen or thigh.
Reconstituted in bacteriostatic water and stored refrigerated at 2–8°C (36–46°F), most peptides remain stable for 4–6 weeks. Some sources cite up to 8 weeks for certain peptides, but degradation accelerates with temperature fluctuations. Never freeze a reconstituted vial. Lyophilized (powder) peptides stored in a freezer at -20°C can remain stable for 12–24 months before reconstitution.
Want to go deeper? Read our complete reconstitution guide for step-by-step photos, storage tips, and injection technique. New to peptides? Start with our beginner's guide.