TB-500 is a research compound. It is not approved by the FDA or any regulatory body for human use. This article is for educational and informational purposes only. Nothing here constitutes medical advice. Consult a qualified physician before considering any peptide use.
TB-500 is typically reconstituted with bacteriostatic water (BAC water). The standard dosage of 2-5 mg (loading), 2 mg (maintenance) is administered 2x weekly (loading phase), weekly (maintenance) via subcutaneous or intramuscular injection. Use our peptide calculator for exact mixing ratios.
How Do You Reconstitute TB-500?
TB-500 is supplied as a lyophilized (freeze-dried) powder that must be reconstituted before use. Proper reconstitution is critical for accurate dosing and maintaining stability.
This guide covers the step-by-step process for mixing TB-500 with bacteriostatic water to achieve your target concentration.
What Supplies Do You Need?
To reconstitute TB-500, you need: the TB-500 vial (lyophilized powder), bacteriostatic water (BAC water with 0.9% benzyl alcohol), insulin syringes (typically 1mL/100-unit), alcohol swabs, and a clean workspace.
Important: Always use bacteriostatic water — not sterile water or saline. BAC water's benzyl alcohol prevents bacterial contamination, extending the usable life of reconstituted TB-500 to 4-6 weeks.
Step-by-Step TB-500 Reconstitution
Step 1: Clean the vial tops of both the TB-500 and BAC water with alcohol swabs.
Step 2: Draw the calculated amount of BAC water into an insulin syringe. Use our peptide calculator to determine the exact amount.
Step 3: Insert the needle into the TB-500 vial at an angle, and let the water run down the side of the glass — never spray directly onto the powder as this can damage the peptide bonds.
Step 4: Gently swirl (do not shake) the vial until the powder is fully dissolved. The solution should be clear.
Step 5: Label the vial with the date and concentration. Store at 2-8°C.
Calculate Your TB-500 Dose
Use our free peptide dosing calculator to get exact reconstitution math and syringe units for TB-500.
Open Calculator →How Do You Dose Reconstituted TB-500?
After reconstitution, the standard TB-500 dose is 2-5 mg (loading), 2 mg (maintenance) administered 2x weekly (loading phase), weekly (maintenance) via subcutaneous or intramuscular injection. The number of units on your insulin syringe depends on how much BAC water you added.
Our calculator will tell you exactly how many units to draw for your dose based on your specific reconstitution ratio.
How Do You Store Reconstituted TB-500?
Store reconstituted TB-500 at 2-8°C (standard refrigerator temperature). Use within 4-6 weeks. Keep away from light and temperature fluctuations.
Unreconstituted TB-500 powder can be stored at -20°C for 12+ months. Once you reconstitute it, the clock starts.
Bottom Line
Reconstituting TB-500 is straightforward — add BAC water, swirl gently, refrigerate. The key is using the right amount of water for accurate dosing. Use our peptide calculator every time.
Complete Guide
TB-500 : Thymosin Beta-4, Research Evidence & Protocols
Related Reading
- TB-500 Dosage Guide
- TB-500 Benefits
- TB-500 Side Effects
- TB-500 Stacking Guide
- TB-500 Cycle Guide
- TB-500 Research
Research-Grade Sourcing
If you're going to research TB-500, source matters. These are the suppliers WolveStack has vetted for purity and third-party testing.
Frequently Asked Questions
What is TB-500?
TB-500 (Thymosin Beta-4 Fragment (TB-500)) is a 43-amino acid peptide. Naturally occurring peptide present in virtually all human and animal cells. It is researched for wound healing, tissue repair, inflammation reduction, hair regrowth, cardiac repair, flexibility improvement.
What is the recommended TB-500 dosage?
Common dosages: 2-5 mg (loading), 2 mg (maintenance) administered 2x weekly (loading phase), weekly (maintenance) via subcutaneous or intramuscular injection. Cycle length: 4-6 weeks loading, then ongoing maintenance. Half-life: approximately 2-3 hours. Use our peptide calculator for exact reconstitution math.
What are the side effects of TB-500?
Generally well-tolerated. Temporary lethargy, head rush, or mild headache reported in some users. Minor injection site irritation possible. No organ toxicity documented in research.
Is TB-500 safe?
TB-500 has shown a favorable safety profile in research. Not FDA-approved. Available as a research chemical. Banned by WADA in athletic competition. All research should follow appropriate safety protocols.