Reconstitution Calculator
Works for BPC-157, TB-500, CJC-1295, Ipamorelin, and any lyophilized peptide
Step-by-Step Instructions
Research use only. These calculations are for educational reference. Peptides are unscheduled research chemicals, not approved medications. Consult a qualified medical professional before use. WolveStack does not provide medical advice.
Common Peptide Reference Doses
Based on community protocols discussed in research forums. Not medical advice.
| Peptide | Common Range | Typical Frequency | Route | Complexity |
|---|---|---|---|---|
| BPC-157 | 200–500 mcg | Once or twice daily | SubQ or IM | Low |
| TB-500 | 2–5 mg | 2× weekly (loading), weekly (maintenance) | SubQ or IM | Low |
| Wolverine Stack | 250 mcg BPC + 2.5 mg TB | BPC daily, TB 2×/week | SubQ | Medium |
| CJC-1295 (no DAC) | 100–200 mcg | 3× daily (pulsed) | SubQ | Medium |
| Ipamorelin | 200–300 mcg | 3× daily (pulsed) | SubQ | Medium |
| Selank | 250–500 mcg | Once or twice daily | Intranasal or SubQ | Low |
| PT-141 | 0.5–2 mg | As needed (1–2 hrs pre) | SubQ | Low |
| AOD-9604 | 300–500 mcg | Once daily (fasted) | SubQ | Low |
| GHK-Cu | 1–3 mg | Once daily | SubQ or topical | Low |
| Epithalon | 5–10 mg | Once daily (cycles) | SubQ or IM | Medium |
Quick Unit Conversion Reference
The mcg → mL math that trips up first-timers.
| If your vial is… | You add 1 mL BAC water | You add 2 mL BAC water | You add 3 mL BAC water |
|---|---|---|---|
| 5 mg vial | 5,000 mcg/mL → 250 mcg = 5 units | 2,500 mcg/mL → 250 mcg = 10 units | 1,667 mcg/mL → 250 mcg = 15 units |
| 10 mg vial | 10,000 mcg/mL → 250 mcg = 2.5 units | 5,000 mcg/mL → 250 mcg = 5 units | 3,333 mcg/mL → 250 mcg = 7.5 units |
| 2 mg vial | 2,000 mcg/mL → 200 mcg = 10 units | 1,000 mcg/mL → 200 mcg = 20 units | 667 mcg/mL → 200 mcg = 30 units |
Calculator FAQ
Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial growth in the vial after you've punctured the stopper. A reconstituted peptide vial can be punctured multiple times safely over 4–6 weeks when stored at 2–8°C in BAC water. Sterile water has no preservative, so a reconstituted vial should ideally be used within 24–48 hours to minimize contamination risk. For multi-use vials, BAC water is the standard choice.
Only in terms of the resulting concentration, which affects how many units you draw per dose. More water = lower concentration = more units per dose. Neither is intrinsically better — the math is what matters. Many people prefer 2 mL per 5 mg vial because it gives round numbers on a U-100 syringe. Use this calculator to find what works for your syringe and dose target.
Both hold 1 mL of liquid, but the graduations differ. A U-100 syringe has 100 units marked on it (each unit = 0.01 mL). A U-50 syringe has 50 units (each unit = 0.02 mL). U-100 syringes are the standard for most peptide protocols because they allow more precise measurement for small doses. The calculator accounts for both — just select yours in the dropdown.
1 mg = 1,000 mcg. So a 5 mg vial contains 5,000 mcg total. When you see peptide doses listed as "250 mcg," that's 0.25 mg — a fraction of the total vial. This matters because vial sizes are typically labeled in mg (e.g., 5 mg, 10 mg) while individual doses are described in mcg (e.g., 250 mcg, 500 mcg). The calculator handles this conversion automatically.
Small air bubbles in subcutaneous (SubQ) injections are generally considered benign — SubQ injections go into fat tissue, not directly into a vein. That said, best practice is to tap the syringe gently and push the plunger slightly to expel visible bubbles before injecting. Air bubbles in intravenous injections are a different matter and pose real risk, but most peptide protocols use SubQ injection into the abdomen or thigh.
Reconstituted in bacteriostatic water and stored refrigerated at 2–8°C (36–46°F), most peptides remain stable for 4–6 weeks. Some sources cite up to 8 weeks for certain peptides, but degradation accelerates with temperature fluctuations. Never freeze a reconstituted vial. Lyophilized (powder) peptides stored in a freezer at -20°C can remain stable for 12–24 months before reconstitution.
Want to go deeper? Read our complete reconstitution guide for step-by-step photos, storage tips, and injection technique. New to peptides? Start with our beginner's guide.