SS-31 is a research compound. It is not approved by the FDA or any regulatory body for human use. This article is for educational and informational purposes only. Nothing here constitutes medical advice. Consult a qualified physician before considering any peptide use.
SS-31 is administered via subcutaneous injection or intravenous infusion at 4-40 mg daily (subcutaneous); 0.01-0.25 mg/kg/hour IV daily subcutaneous or weekly IV infusion. Subcutaneous injections into the abdominal fat or thigh are most common. Proper reconstitution with bacteriostatic water is required first.
How Do You Inject SS-31?
SS-31 is administered via subcutaneous injection or intravenous infusion. For most researchers, subcutaneous injection is the standard approach — it's simple, relatively painless, and effective for Mitochondrial-targeting tetrapeptide compounds.
This guide covers injection technique, site selection, needle choices, and common mistakes.
How Do You Prepare for a SS-31 Injection?
Step 1: Wash your hands thoroughly.
Step 2: Clean the top of the SS-31 vial and BAC water vial with alcohol swabs. If not yet reconstituted, see our SS-31 reconstitution guide.
Step 3: Draw your dose (4-40 mg daily (subcutaneous); 0.01-0.25 mg/kg/hour IV) into an insulin syringe. Use our calculator for exact units.
Step 4: Clean the injection site with an alcohol swab and let it dry.
What Is the Correct Injection Technique?
Subcutaneous (most common): Pinch a fold of skin — typically abdominal fat 2+ inches from the navel, or the thigh. Insert the needle at a 45-degree angle. Push the plunger slowly and steadily. Hold for 5 seconds, then withdraw.
Intramuscular (less common for SS-31): Insert the needle at 90 degrees into the muscle (deltoid or vastus lateralis). This route provides faster absorption but isn't necessary for most peptide protocols.
Rotate injection sites to prevent lipodystrophy (fat tissue changes from repeated injections in the same spot).
What Size Needle Should You Use?
For subcutaneous SS-31 injections, 29-31 gauge insulin needles (½ inch or 8mm) are standard. These are thin enough to be nearly painless while long enough for proper subcutaneous delivery.
Use a fresh needle for every injection. Never reuse or share needles.
Calculate Your SS-31 Dose
Use our free peptide dosing calculator to get exact reconstitution math and syringe units for SS-31.
Open Calculator →What Are Common Injection Side Effects?
Mild redness, swelling, or itching at the injection site is normal and typically resolves within hours. Small bruises can occur, especially if you hit a capillary.
If you experience persistent pain, swelling, warmth, or redness lasting more than 24 hours, discontinue and consult a healthcare provider — these may indicate infection.
Bottom Line on SS-31 Injection
SS-31 is administered via subcutaneous injection or intravenous infusion at 4-40 mg daily (subcutaneous); 0.01-0.25 mg/kg/hour IV daily subcutaneous or weekly IV infusion. Subcutaneous injection with a 29-31 gauge insulin needle into abdominal fat is the standard technique. Rotate sites and use a fresh needle every time.
Complete Guide
SS-31 (Elamipretide) : Mitochondrial Peptide for Energy & Aging
Related Reading
- SS-31 Dosage Guide
- SS-31 Benefits
- SS-31 Side Effects
- SS-31 Stacking Guide
- SS-31 Cycle Guide
- SS-31 Research
Research-Grade Sourcing
If you're going to research SS-31, source matters. These are the suppliers WolveStack has vetted for purity and third-party testing.
Frequently Asked Questions
What is SS-31?
SS-31 (Elamipretide (SS-31)) is a Mitochondrial-targeting tetrapeptide. Synthetic tetrapeptide co-discovered for mitochondrial dysfunction disorders; developed by Stealth BioTherapeutics. It is researched for improved muscle strength, enhanced cardiac function, increased ATP production, reduced oxidative stress, restored age-related mitochondrial dysfunction.
What is the recommended SS-31 dosage?
Common dosages: 4-40 mg daily (subcutaneous); 0.01-0.25 mg/kg/hour IV administered daily subcutaneous or weekly IV infusion via subcutaneous injection or intravenous infusion. Cycle length: 12-48 weeks in clinical trials. Half-life: approximately 20-30 minutes. Use our peptide calculator for exact reconstitution math.
What are the side effects of SS-31?
Excellent safety profile. No adverse events reported in single-dose IV studies. Well-tolerated across wide dose ranges in long-term Phase 2 and 3 trials.
Is SS-31 safe?
SS-31 has shown a favorable safety profile in research. FDA-approved September 2025 (FORZINITY) for Barth syndrome. Phase 3 trials ongoing for other indications. All research should follow appropriate safety protocols.