Humanin is a research compound. It is not approved by the FDA or any regulatory body for human use. This article is for educational and informational purposes only. Nothing here constitutes medical advice. Consult a qualified physician before considering any peptide use.
Humanin is typically reconstituted with bacteriostatic water (BAC water). The standard dosage of 2.5 mg/kg (animal studies); human dosing not established is administered twice daily in animal protocols via subcutaneous injection (proposed for humans). Use our peptide calculator for exact mixing ratios.
How Do You Reconstitute Humanin?
Humanin is supplied as a lyophilized (freeze-dried) powder that must be reconstituted before use. Proper reconstitution is critical for accurate dosing and maintaining stability.
This guide covers the step-by-step process for mixing Humanin with bacteriostatic water to achieve your target concentration.
What Supplies Do You Need?
To reconstitute Humanin, you need: the Humanin vial (lyophilized powder), bacteriostatic water (BAC water with 0.9% benzyl alcohol), insulin syringes (typically 1mL/100-unit), alcohol swabs, and a clean workspace.
Important: Always use bacteriostatic water — not sterile water or saline. BAC water's benzyl alcohol prevents bacterial contamination, extending the usable life of reconstituted Humanin to 4-6 weeks.
Step-by-Step Humanin Reconstitution
Step 1: Clean the vial tops of both the Humanin and BAC water with alcohol swabs.
Step 2: Draw the calculated amount of BAC water into an insulin syringe. Use our peptide calculator to determine the exact amount.
Step 3: Insert the needle into the Humanin vial at an angle, and let the water run down the side of the glass — never spray directly onto the powder as this can damage the peptide bonds.
Step 4: Gently swirl (do not shake) the vial until the powder is fully dissolved. The solution should be clear.
Step 5: Label the vial with the date and concentration. Store at 2-8°C.
Calculate Your Humanin Dose
Use our free peptide dosing calculator to get exact reconstitution math and syringe units for Humanin.
Open Calculator →How Do You Dose Reconstituted Humanin?
After reconstitution, the standard Humanin dose is 2.5 mg/kg (animal studies); human dosing not established administered twice daily in animal protocols via subcutaneous injection (proposed for humans). The number of units on your insulin syringe depends on how much BAC water you added.
Our calculator will tell you exactly how many units to draw for your dose based on your specific reconstitution ratio.
How Do You Store Reconstituted Humanin?
Store reconstituted Humanin at 2-8°C (standard refrigerator temperature). Use within 4-6 weeks. Keep away from light and temperature fluctuations.
Unreconstituted Humanin powder can be stored at -20°C for 12+ months. Once you reconstitute it, the clock starts.
Bottom Line
Reconstituting Humanin is straightforward — add BAC water, swirl gently, refrigerate. The key is using the right amount of water for accurate dosing. Use our peptide calculator every time.
Complete Guide
Humanin: The Mitochondrial Peptide
Related Reading
- Humanin Dosage Guide
- Humanin Benefits
- Humanin Side Effects
- Humanin Stacking Guide
- Humanin Cycle Guide
- Humanin Research
Research-Grade Sourcing
If you're going to research Humanin, source matters. These are the suppliers WolveStack has vetted for purity and third-party testing.
Frequently Asked Questions
What is Humanin?
Humanin (Humanin mitochondrial-derived peptide) is a Mitochondrial-derived peptide, cytoprotective factor. Endogenous 24-amino acid peptide encoded within mitochondrial DNA, released under stress conditions as a survival signal. It is researched for neuroprotection, Alzheimer's disease mitigation, cognitive preservation, anti-aging, metabolic health, mitochondrial restoration.
What is the recommended Humanin dosage?
Common dosages: 2.5 mg/kg (animal studies); human dosing not established administered twice daily in animal protocols via subcutaneous injection (proposed for humans). Cycle length: human protocol undefined; chronic dosing in animal models. Half-life: ~30 minutes in mice; >4 hours in rats. Use our peptide calculator for exact reconstitution math.
What are the side effects of Humanin?
No significant side effects in preclinical studies. Human safety data completely absent. Potential for antibody formation with chronic use.
Is Humanin safe?
Humanin has shown a preliminary safety profile in research. Not FDA-approved. Research peptide only. All research should follow appropriate safety protocols.