SS-31 is a research compound. It is not approved by the FDA or any regulatory body for human use. This article is for educational and informational purposes only. Nothing here constitutes medical advice. Consult a qualified physician before considering any peptide use.
SS-31 is typically reconstituted with bacteriostatic water (BAC water). The standard dosage of 4-40 mg daily (subcutaneous); 0.01-0.25 mg/kg/hour IV is administered daily subcutaneous or weekly IV infusion via subcutaneous injection or intravenous infusion. Use our peptide calculator for exact mixing ratios.
How Do You Reconstitute SS-31?
SS-31 is supplied as a lyophilized (freeze-dried) powder that must be reconstituted before use. Proper reconstitution is critical for accurate dosing and maintaining stability.
This guide covers the step-by-step process for mixing SS-31 with bacteriostatic water to achieve your target concentration.
What Supplies Do You Need?
To reconstitute SS-31, you need: the SS-31 vial (lyophilized powder), bacteriostatic water (BAC water with 0.9% benzyl alcohol), insulin syringes (typically 1mL/100-unit), alcohol swabs, and a clean workspace.
Important: Always use bacteriostatic water — not sterile water or saline. BAC water's benzyl alcohol prevents bacterial contamination, extending the usable life of reconstituted SS-31 to 4-6 weeks.
Step-by-Step SS-31 Reconstitution
Step 1: Clean the vial tops of both the SS-31 and BAC water with alcohol swabs.
Step 2: Draw the calculated amount of BAC water into an insulin syringe. Use our peptide calculator to determine the exact amount.
Step 3: Insert the needle into the SS-31 vial at an angle, and let the water run down the side of the glass — never spray directly onto the powder as this can damage the peptide bonds.
Step 4: Gently swirl (do not shake) the vial until the powder is fully dissolved. The solution should be clear.
Step 5: Label the vial with the date and concentration. Store at 2-8°C.
Calculate Your SS-31 Dose
Use our free peptide dosing calculator to get exact reconstitution math and syringe units for SS-31.
Open Calculator →How Do You Dose Reconstituted SS-31?
After reconstitution, the standard SS-31 dose is 4-40 mg daily (subcutaneous); 0.01-0.25 mg/kg/hour IV administered daily subcutaneous or weekly IV infusion via subcutaneous injection or intravenous infusion. The number of units on your insulin syringe depends on how much BAC water you added.
Our calculator will tell you exactly how many units to draw for your dose based on your specific reconstitution ratio.
How Do You Store Reconstituted SS-31?
Store reconstituted SS-31 at 2-8°C (standard refrigerator temperature). Use within 4-6 weeks. Keep away from light and temperature fluctuations.
Unreconstituted SS-31 powder can be stored at -20°C for 12+ months. Once you reconstitute it, the clock starts.
Bottom Line
Reconstituting SS-31 is straightforward — add BAC water, swirl gently, refrigerate. The key is using the right amount of water for accurate dosing. Use our peptide calculator every time.
Complete Guide
SS-31 (Elamipretide) : Mitochondrial Peptide for Energy & Aging
Related Reading
- SS-31 Dosage Guide
- SS-31 Benefits
- SS-31 Side Effects
- SS-31 Stacking Guide
- SS-31 Cycle Guide
- SS-31 Research
Research-Grade Sourcing
If you're going to research SS-31, source matters. These are the suppliers WolveStack has vetted for purity and third-party testing.
Frequently Asked Questions
What is SS-31?
SS-31 (Elamipretide (SS-31)) is a Mitochondrial-targeting tetrapeptide. Synthetic tetrapeptide co-discovered for mitochondrial dysfunction disorders; developed by Stealth BioTherapeutics. It is researched for improved muscle strength, enhanced cardiac function, increased ATP production, reduced oxidative stress, restored age-related mitochondrial dysfunction.
What is the recommended SS-31 dosage?
Common dosages: 4-40 mg daily (subcutaneous); 0.01-0.25 mg/kg/hour IV administered daily subcutaneous or weekly IV infusion via subcutaneous injection or intravenous infusion. Cycle length: 12-48 weeks in clinical trials. Half-life: approximately 20-30 minutes. Use our peptide calculator for exact reconstitution math.
What are the side effects of SS-31?
Excellent safety profile. No adverse events reported in single-dose IV studies. Well-tolerated across wide dose ranges in long-term Phase 2 and 3 trials.
Is SS-31 safe?
SS-31 has shown a favorable safety profile in research. FDA-approved September 2025 (FORZINITY) for Barth syndrome. Phase 3 trials ongoing for other indications. All research should follow appropriate safety protocols.